Hotspot

rpl32-trnL-UAG

SSC · 117408-118898 · 1491 bp

Back to Rhaphiolepis

Pi 0.00302
MarkerSeek score 56.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1491
variable_sites
11
pi
0.00302
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1567-1606 bp 1.0 81.2
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1568-1607 bp 1.0 81.2
rpl32-trnL-UAG_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1569-1608 bp 1.0 80.8
rpl32-trnL-UAG_p4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1570-1609 bp 1.0 80.8
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1577-1616 bp 1.0 80.3