Hotspot

ndhF-rpl32

SSC · 116171-117248 · 1078 bp

Back to Rhaphiolepis

Pi 0.00434
MarkerSeek score 56.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1078
variable_sites
11
pi
0.00434
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGT AAAAGCTTTCAATGCTGCCC 45.0 45.0 55.2 57.8 1193-1269 bp 1.0 59.5
ndhF-rpl32_p2 CTGGAAGTGGAATGAAAGGT AAGCTTTCAATGCTGCCCTA 45.0 45.0 55.2 57.8 1191-1267 bp 1.0 59.1
ndhF-rpl32_p3 CTGGAAGTGGAATGAAAGGT GCTGCCCTATATCCCTTCCT 45.0 55.0 55.2 58.3 1180-1256 bp 1.0 58.2
ndhF-rpl32_p4 CTGGAAGTGGAATGAAAGGT AGCTTTCAATGCTGCCCTAT 45.0 45.0 55.2 57.6 1190-1266 bp 1.0 57.3
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGT TGCTGCCCTATATCCCTTCC 45.0 55.0 55.2 58.6 1181-1257 bp 1.0 55.8