Hotspot

psbZ-trnG-GCC

LSC · 38380-39127 · 748 bp

Back to Rhaphiolepis

Pi 0.00604

MarkerSeek score 53.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 5
pi: 0.00604
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	619-906 bp	1.0	81.0
psbZ-trnG-GCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	605-892 bp	1.0	80.8
psbZ-trnG-GCC_p3	TTTGCTTCTCCTGAGGGTTG	TTTATCCATTACCCTGGGCG	50.0	50.0	57.7	57.4	659-946 bp	1.0	77.1
psbZ-trnG-GCC_p4	GGGTTGGTCGGGTAACAAAA	TTTATCCATTACCCTGGGCG	50.0	50.0	58.3	57.4	645-932 bp	1.0	76.9
psbZ-trnG-GCC_p5	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	575-862 bp	1.0	75.1