Hotspot

trnC-GCA-petN

LSC · 28764-29743 · 980 bp

Back to Rhaphiolepis

Pi 0.00157

MarkerSeek score 47.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 4
pi: 0.00157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	1017-1037 bp	1.0	81.0
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	57.9	58.0	1069-1089 bp	1.0	80.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.1	58.1	1075-1095 bp	1.0	80.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	GTCCACTCCTTCCCCATACT	50.0	55.0	58.1	58.1	1074-1094 bp	1.0	80.2
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	GTCCACTCCTTCCCCATACT	50.0	55.0	57.9	58.1	1126-1146 bp	1.0	79.6