Hotspot

atpH-atpI

LSC · 14010-15183 · 1174 bp

Back to Rhaphiolepis

Pi 0.000426

MarkerSeek score 52.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 2
pi: 0.000426
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1262-1268 bp	1.0	80.1
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1228-1234 bp	1.0	80.1
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1292-1298 bp	1.0	79.3
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1258-1264 bp	1.0	79.2
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1229-1235 bp	1.0	78.9