Hotspot

ndhF

SSC · 115977-118229 · 2253 bp

Back to Rhamnus

Pi 0.00449

MarkerSeek score 46.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 26
pi: 0.00449
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AACCGACCCCTTTCAAAAGA	50.0	45.0	58.0	57.3	2559-2610 bp	1.0	75.2
ndhF_p2	ACGAAGGGGACTAAACAGGA	ACCGACCCCTTTCAAAAGAA	50.0	45.0	58.0	57.3	2558-2609 bp	1.0	75.2
ndhF_p3	ACAGGAACAAGAGGGATCCA	AACCGACCCCTTTCAAAAGA	50.0	45.0	58.0	57.3	2545-2596 bp	1.0	75.0
ndhF_p4	ACAGGAACAAGAGGGATCCA	ACCGACCCCTTTCAAAAGAA	50.0	45.0	58.0	57.3	2544-2595 bp	1.0	75.0
ndhF_p5	AAGAGGGATCCACCGAAGAA	ACCGACCCCTTTCAAAAGAA	50.0	45.0	58.0	57.3	2536-2587 bp	1.0	75.0