Hotspot

rpl32-trnL-UAG

SSC · 119012-120436 · 1425 bp

Back to Rhamnus

Pi 0.00668
MarkerSeek score 57.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1425
variable_sites
26
pi
0.00668
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGAAAGCTTTTTCGTTAGCGA AGCGTGTCTACCGATTTCAC 38.1 50.0 56.8 58.0 1520-1546 bp 1.0 64.4
rpl32-trnL-UAG_p2 TGAAAGCTTTTTCGTTAGCGA CAGCGTGTCTACCGATTTCA 38.1 50.0 56.8 58.0 1521-1547 bp 1.0 64.4
rpl32-trnL-UAG_p3 TGAAAGCTTTTTCGTTAGCGA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 56.8 57.9 1530-1556 bp 1.0 63.4
rpl32-trnL-UAG_p4 TGAAAGCTTTTTCGTTAGCGA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 56.8 57.8 1529-1555 bp 1.0 62.9
rpl32-trnL-UAG_p5 TGAAAGCTTTTTCGTTAGCGA CTAAGAGCAGCGTGTCTACC 38.1 55.0 56.8 57.8 1528-1554 bp 1.0 62.9