Hotspot

ndhF-rpl32

SSC · 118230-118852 · 623 bp

Back to Rhamnus

Pi 0.00887
MarkerSeek score 57.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
623
variable_sites
17
pi
0.00887
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGTTGGAAGGGGAATGAAA TGATATAGAGGTGCGTTTTT 50.0 35.0 57.7 52.3 710-723 bp 1.0 42.5
ndhF-rpl32_p2 GGGTTGGAAGGGGAATGAAA TTGATATAGAGGTGCGTTTT 50.0 35.0 57.7 52.3 711-724 bp 1.0 42.5
ndhF-rpl32_p3 GGGTTGGAAGGGGAATGAAA TTTGATATAGAGGTGCGTTT 50.0 35.0 57.7 52.3 712-725 bp 1.0 42.5
ndhF-rpl32_p4 GGGTTGGAAGGGGAATGAAA TTTTGATATAGAGGTGCGTT 50.0 35.0 57.7 52.3 713-726 bp 1.0 42.5
ndhF-rpl32_p5 GGGTTGGAAGGGGAATGAAA TTTTTGATATAGAGGTGCGT 50.0 35.0 57.7 52.3 714-727 bp 1.0 42.4