Hotspot

rps19-rpl2

LSC · 89633-89755 · 123 bp

Back to Rhamnus

Pi 0.116

MarkerSeek score 60.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 50
pi: 0.116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCAATAGTATGCCCGATCAT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	55.5	58.1	262-337 bp	1.0	67.4
rps19-rpl2_p2	GCAATAGTATGCCCGATCAT	TTGGGGTTATCCTGCACTTG	45.0	50.0	55.5	57.8	228-303 bp	1.0	66.2
rps19-rpl2_p3	GCAATAGTATGCCCGATCAT	ATTTGATTCTTCGTCGCCGT	45.0	45.0	55.5	58.3	172-247 bp	1.0	65.7
rps19-rpl2_p4	GCAATAGTATGCCCGATCAT	TTGATTCTTCGTCGCCGTAA	45.0	45.0	55.5	57.6	170-245 bp	1.0	65.2
rps19-rpl2_p5	GCAATAGTATGCCCGATCAT	TTTGATTCTTCGTCGCCGTA	45.0	45.0	55.5	57.6	171-246 bp	1.0	65.2