Hotspot

psbE-petL

LSC · 69454-70847 · 1394 bp

Back to Rhamnus

Pi 0.00444

MarkerSeek score 58.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1394
variable_sites: 18
pi: 0.00444
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CCCGATAAATAGAGCCGAGG	50.0	55.0	57.9	57.3	1488-1580 bp	1.0	79.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CCCGATAAATAGAGCCGAGG	50.0	55.0	57.8	57.3	1411-1503 bp	1.0	78.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	CCCGATAAATAGAGCCGAGG	45.0	55.0	58.2	57.3	1497-1589 bp	1.0	78.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	CCCGATAAATAGAGCCGAGG	50.0	55.0	58.2	57.3	1502-1594 bp	1.0	78.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	CCCGATAAATAGAGCCGAGG	50.0	55.0	57.8	57.3	1496-1588 bp	1.0	78.2