Hotspot

accD-psaI

LSC · 63306-64046 · 741 bp

Back to Rhamnus

Pi 0.00546

MarkerSeek score 63.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 12
pi: 0.00546
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	858-902 bp	1.0	81.9
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.4	58.0	849-893 bp	1.0	81.7
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	AGAAACCATTGCAATTGCCG	50.0	45.0	58.4	57.9	863-907 bp	1.0	81.4
accD-psaI_p4	GGGCGTTCTGAGTGAGTTAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	857-901 bp	1.0	79.5
accD-psaI_p5	GGGCGTTCTGAGTGAGTTAT	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.3	58.0	848-892 bp	1.0	79.3