Hotspot

ndhC-trnV-UAC

LSC · 55095-55616 · 522 bp

Back to Rhamnus

Pi 0.00693

MarkerSeek score 61.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 11
pi: 0.00693
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	651-658 bp	1.0	80.0
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCACAAAAATGC	50.0	45.0	57.9	58.5	730-737 bp	1.0	76.8
ndhC-trnV-UAC_p3	TTTTCTGGTCCTTTGCGCAT	CCGAGAAAGTCTACGGTTCG	45.0	55.0	58.7	58.1	672-679 bp	1.0	75.6
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	636-643 bp	1.0	75.4
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	GAAAGAGCCCAATGCACAAA	50.0	45.0	57.9	57.2	735-742 bp	1.0	74.1