Hotspot

psbZ-trnG-GCC

LSC · 39179-39741 · 563 bp

Back to Rhamnus

Pi 0.00813

MarkerSeek score 66.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 12
pi: 0.00813
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	730-751 bp	1.0	80.3
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	731-752 bp	1.0	80.3
psbZ-trnG-GCC_p3	CTGATGGTTGGGCGGATAAT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	721-742 bp	1.0	79.7
psbZ-trnG-GCC_p4	CTGATGGTTGGGCGGATAAT	GAATCGAACCCGCATCTTCT	50.0	50.0	57.7	58.1	722-743 bp	1.0	79.7
psbZ-trnG-GCC_p5	CCGTCGTATTTGCTTCTCCT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	739-760 bp	1.0	79.5