Hotspot

trnD-GUC-trnY-GUA

LSC · 32970-33431 · 462 bp

Back to Rhamnus

Pi 0.00557

MarkerSeek score 61.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 8
pi: 0.00557
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTGGATTTGGATCGGTCGG	AATTGATTCTTCCCGGGTCG	50.0	50.0	58.0	58.0	648-654 bp	1.0	80.8
trnD-GUC-trnY-GUA_p2	ATTGGATTTGGATCGGTCGG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	590-596 bp	1.0	79.3
trnD-GUC-trnY-GUA_p3	ATTGGATTTGGATCGGTCGG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	589-595 bp	1.0	79.3
trnD-GUC-trnY-GUA_p4	ATTGGATTTGGATCGGTCGG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	611-617 bp	1.0	78.4
trnD-GUC-trnY-GUA_p5	ATTGGATTTGGATCGGTCGG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	610-616 bp	1.0	78.2