Hotspot

trnC-GCA-petN

LSC · 30052-31007 · 956 bp

Back to Rhamnus

Pi 0.00578

MarkerSeek score 56.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 16
pi: 0.00578
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1004-1012 bp	1.0	80.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1065-1073 bp	1.0	78.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1022-1030 bp	1.0	77.8
trnC-GCA-petN_p4	CTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1060-1068 bp	1.0	77.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	1083-1091 bp	1.0	76.1