Hotspot

atpH-atpI

LSC · 15265-16476 · 1212 bp

Back to Rhamnus

Pi 0.00118
MarkerSeek score 62.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1212
variable_sites
5
pi
0.00118
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 1248-1273 bp 1.0 81.9
atpH-atpI_p2 GATCCCTTCTACAGCTTGGC GCGAATCCATGGAAGGTCAT 55.0 50.0 58.0 58.0 1333-1358 bp 1.0 81.7
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT AACTTTGGCTGCGGCTTATA 45.0 45.0 58.0 57.9 1271-1296 bp 1.0 81.1
atpH-atpI_p4 GATCCCTTCTACAGCTTGGC AACTTTGGCTGCGGCTTATA 55.0 45.0 58.0 57.9 1356-1381 bp 1.0 80.9
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 1249-1274 bp 1.0 80.7