Hotspot

ndhF-rpl32

SSC · 117779-118693 · 915 bp

Back to Rhamnella

Pi 0.0369

MarkerSeek score 70.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 915
variable_sites: 54
pi: 0.0369
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTTAGGGCCGTCGGAAAAA	CGAGCCGGACCAATTTCTAT	45.0	50.0	58.0	57.8	747-1211 bp	0.5	63.9
ndhF-rpl32_p2	TTTTTAGGGCCGTCGGAAAA	CGAGCCGGACCAATTTCTAT	45.0	50.0	58.0	57.8	748-1212 bp	0.5	63.9
ndhF-rpl32_p3	ATCCCCGATCATGAGCCATA	CGAGCCGGACCAATTTCTAT	50.0	50.0	58.0	57.8	1006-1470 bp	0.5	63.7
ndhF-rpl32_p4	TATCCCCGATCATGAGCCAT	CGAGCCGGACCAATTTCTAT	50.0	50.0	58.0	57.8	1007-1471 bp	0.5	63.7
ndhF-rpl32_p5	TCCCCGATCATGAGCCATAT	CGAGCCGGACCAATTTCTAT	50.0	50.0	58.0	57.8	1005-1469 bp	0.5	63.7