Hotspot

rpl22-rps19

IRb · 88747-88848 · 102 bp

Back to Rhamnella

Pi 0.0667
MarkerSeek score 77.50
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
102
variable_sites
12
pi
0.0667
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.88
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCGGTTCTTTTTGCTCTTCT GGGGCATGCCAAAAATGATA 40.0 45.0 55.2 56.7 154-166 bp 1.0 57.4
rpl22-rps19_p2 TCGGTTCTTTTTGCTCTTCT GGGGGCATGCCAAAAATGAT 40.0 50.0 55.2 59.5 155-167 bp 1.0 56.1
rpl22-rps19_p3 TCGGTTCTTTTTGCTCTTCTT GGGGCATGCCAAAAATGATA 38.1 45.0 56.0 56.7 154-166 bp 1.0 55.8
rpl22-rps19_p4 TCGGTTCTTTTTGCTCTTCT GGGGCATGCCAAAAATGATAA 40.0 42.9 55.2 57.4 154-166 bp 1.0 55.3
rpl22-rps19_p5 TCGGTTCTTTTTGCTCTTCTT GGGGGCATGCCAAAAATGAT 38.1 50.0 56.0 59.5 155-167 bp 1.0 54.5