Hotspot

petA-psbJ

LSC · 66996-68057 · 1062 bp

Back to Rhamnella

Pi 0.0785

MarkerSeek score 76.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 131
pi: 0.0785
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTTCAATTGTCCGAAATGAA	TCTTGTGATCGGTTTAATCGGT	35.0	40.9	52.4	57.8	616-1169 bp	1.0	52.0
petA-psbJ_p2	GTTCAATTGTCCGAAATGAA	ACTGGTATTCTTGTGATCGGT	35.0	42.9	52.4	56.7	624-1177 bp	1.0	52.0
petA-psbJ_p3	TCAATTGTCCGAAATGAATTTCT	TCTTGTGATCGGTTTAATCGGT	30.4	40.9	55.1	57.8	614-1167 bp	1.0	52.0
petA-psbJ_p4	GTTCAATTGTCCGAAATGAATTTCT	TCTTGTGATCGGTTTAATCGGT	32.0	40.9	57.1	57.8	616-1169 bp	1.0	52.0
petA-psbJ_p5	GTTCAATTGTCCGAAATGAAT	TCTTGTGATCGGTTTAATCGGT	33.3	40.9	53.1	57.8	616-1169 bp	1.0	52.0