Hotspot

trnF-GAA-ndhJ

LSC · 52168-52918 · 751 bp

Back to Rhamnella

Pi 0.0215

MarkerSeek score 75.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 30
pi: 0.0215
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	913-938 bp	1.0	84.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	877-902 bp	1.0	84.5
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	901-926 bp	1.0	83.6
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	926-951 bp	1.0	83.5
trnF-GAA-ndhJ_p5	GTGAATGGTCGGGATAGCTC	ATCCACGCCTGAAACGTATC	55.0	50.0	57.8	58.1	923-948 bp	1.0	83.4