Hotspot

rps4-trnT-UGU

LSC · 49349-49827 · 479 bp

Back to Rhamnella

Pi 0.027

MarkerSeek score 74.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 23
pi: 0.027
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	589-607 bp	1.0	85.0
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	593-611 bp	1.0	84.6
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	GGGGTTAGAGCATCGCATTT	50.0	50.0	58.0	58.3	617-635 bp	1.0	84.2
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	GGGGTTAGAGCATCGCATTT	50.0	50.0	58.1	58.3	621-639 bp	1.0	83.8
rps4-trnT-UGU_p5	GCTTCCGGCTCTAGGTTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	582-600 bp	1.0	83.8