Hotspot

trnD-GUC-trnY-GUA

LSC · 32607-33060 · 454 bp

Back to Rhamnella

Pi 0.0253

MarkerSeek score 70.90

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 22
pi: 0.0253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTGGATTTGGATCGGTCGG	AATTGATTCTTCCGGGGTCG	50.0	50.0	58.0	58.0	621-650 bp	1.0	85.8
trnD-GUC-trnY-GUA_p2	ATTGGATTTGGATCGGTCGG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	563-592 bp	1.0	84.9
trnD-GUC-trnY-GUA_p3	ATTGGATTTGGATCGGTCGG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	562-591 bp	1.0	84.9
trnD-GUC-trnY-GUA_p4	ATTGGATTTGGATCGGTCGG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	584-613 bp	1.0	83.7
trnD-GUC-trnY-GUA_p5	ATTGGATTTGGATCGGTCGG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	583-612 bp	1.0	83.5