Hotspot

trnR-UCU-atpA

LSC · 10896-11215 · 320 bp

Back to Rhamnella

Pi 0.0466

MarkerSeek score 80.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 26
pi: 0.0466
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	GAGGAAGCAGAAGCCCTTTT	42.9	50.0	57.4	58.1	418-472 bp	1.0	75.3
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	GAGGAAGCAGAAGCCCTTTT	42.9	50.0	57.4	58.1	417-471 bp	1.0	75.3
trnR-UCU-atpA_p3	TGGAATGAAAAGCGTCCATTG	TTAACGAGGAAGCAGAAGCC	42.9	50.0	57.4	57.9	423-477 bp	1.0	75.2
trnR-UCU-atpA_p4	GGAATGAAAAGCGTCCATTGT	TTAACGAGGAAGCAGAAGCC	42.9	50.0	57.4	57.9	422-476 bp	1.0	75.2
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	357-411 bp	1.0	75.1