Hotspot

trnS-GCU-trnG-UCC

LSC · 8665-9794 · 1130 bp

Back to Rhamnella

Pi 0.0341
MarkerSeek score 71.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1130
variable_sites
65
pi
0.0341
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1199-1245 bp 1.0 59.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1198-1244 bp 1.0 59.9
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1201-1247 bp 1.0 58.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1200-1246 bp 1.0 58.8
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 1253-1299 bp 1.0 58.6