Hotspot

trnH-GUG-psbA

LSC · 95-420 · 326 bp

Back to Rhamnella

Pi 0.0514

MarkerSeek score 77.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 29
pi: 0.0514
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTGTCGACGTTCCATCTACA	55.0	50.0	58.1	57.1	354-379 bp	1.0	82.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CTGTCGACGTTCCATCTACA	50.0	50.0	57.9	57.1	368-393 bp	1.0	81.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CTGTCGACGTTCCATCTACA	50.0	50.0	57.9	57.1	367-392 bp	1.0	81.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTGTCGACGTTCCATCTACA	50.0	50.0	57.9	57.1	369-394 bp	1.0	81.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CTGTCGACGTTCCATCTACAA	55.0	47.6	58.1	57.8	354-379 bp	1.0	79.6