Hotspot

ccsA-ndhD

SSC · 115030-115246 · 217 bp

Back to Rhammatophyllum

Pi 0.0586
MarkerSeek score 78.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
217
variable_sites
18
pi
0.0586
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGTTATGGTTCATTTACATCGA GACAAGGTCGAATCCATTTT 31.8 40.0 53.3 53.9 273-286 bp 1.0 49.1
ccsA-ndhD_p2 AGTTATGGTTCATTTACATCGAA GACAAGGTCGAATCCATTTT 30.4 40.0 54.1 53.9 273-286 bp 1.0 49.1
ccsA-ndhD_p3 AGTTATGGTTCATTTACATCGAAT GACAAGGTCGAATCCATTTT 29.2 40.0 54.7 53.9 273-286 bp 1.0 49.1
ccsA-ndhD_p4 GTTATGGTTCATTTACATCGAA GACAAGGTCGAATCCATTTT 31.8 40.0 52.6 53.9 272-285 bp 1.0 49.1
ccsA-ndhD_p5 ACATAGTTATGGTTCATTTACATCGA GACAAGGTCGAATCCATTTT 30.8 40.0 56.8 53.9 277-290 bp 1.0 49.0