Hotspot

rpl16-rps3

LSC · 81885-82059 · 175 bp

Back to Rhammatophyllum

Pi 0.0476

MarkerSeek score 71.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 12
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	AGAGAAGGCAGAGTTCCTTT	45.0	45.0	57.1	56.1	315-316 bp	1.0	60.7
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	AGGCAGAGTTCCTTTACAAACA	45.0	40.9	57.1	57.8	310-311 bp	1.0	59.0
rpl16-rps3_p3	ACGAGTCACACACTAAGCAT	AGAGAAGGCAGAGTTCCTTT	45.0	45.0	56.9	56.1	314-315 bp	1.0	58.9
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	GAGAAGGCAGAGTTCCTTTACA	45.0	45.5	57.1	57.7	314-315 bp	1.0	58.1
rpl16-rps3_p5	ACGAGTCACACACTAAGCAT	AGGCAGAGTTCCTTTACAAACA	45.0	40.9	56.9	57.8	309-310 bp	1.0	57.2