Hotspot

rps12-clpP

LSC · 69042-69223 · 182 bp

Back to Rhammatophyllum

Pi 0.0476

MarkerSeek score 74.80

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 182
variable_sites: 13
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGGATTGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	295 bp	1.0	81.0
rps12-clpP_p2	CGAGTACATGTTCCTCGTCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.2	57.9	347 bp	1.0	80.6
rps12-clpP_p3	CCGAGTACATGTTCCTCGTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.8	57.9	348 bp	1.0	80.6
rps12-clpP_p4	TTTCGGATTGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	290 bp	1.0	80.5
rps12-clpP_p5	CACCCGAGTACATGTTCCTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.7	57.9	351 bp	1.0	79.7