Hotspot

psaJ-rpl33

LSC · 66369-66794 · 426 bp

Back to Rhammatophyllum

Pi 0.0377

MarkerSeek score 81.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 23
pi: 0.0377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTAGCGCC	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.8	57.8	637-642 bp	1.0	81.4
psaJ-rpl33_p2	TAGCGCCGGTACTAAGTACT	ATATCTGGAAATTCCCGCCG	50.0	50.0	57.7	57.8	624-629 bp	1.0	80.1
psaJ-rpl33_p3	ACATATCTTTCCGTAGCGCC	AATTCCCGCCGATTCTTTCT	50.0	45.0	57.8	57.6	628-633 bp	1.0	79.6
psaJ-rpl33_p4	ACATATCTTTCCGTAGCGCC	ATTCCCGCCGATTCTTTCTT	50.0	45.0	57.8	57.6	627-632 bp	1.0	79.6
psaJ-rpl33_p5	GTAGCGCCGGTACTAAGTAC	ATATCTGGAAATTCCCGCCG	55.0	50.0	57.6	57.8	625-630 bp	1.0	79.6