Hotspot

petA-psbJ

LSC · 62055-62965 · 911 bp

Back to Rhammatophyllum

Pi 0.0242

MarkerSeek score 69.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 32
pi: 0.0242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1089-1102 bp	1.0	71.6
petA-psbJ_p2	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1090-1103 bp	1.0	70.9
petA-psbJ_p3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.4	58.1	1063-1076 bp	1.0	70.4
petA-psbJ_p4	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.4	57.8	1064-1077 bp	1.0	69.7
petA-psbJ_p5	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGG	45.0	50.0	57.6	56.3	1089-1102 bp	1.0	66.8