Hotspot

trnL-UUA-trnF-UUC

LSC · 46944-47609 · 666 bp

Back to Rhammatophyllum

Pi 0.0337

MarkerSeek score 74.70

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 29
pi: 0.0337
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	679-794 bp	1.0	82.9
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	676-791 bp	1.0	79.7
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	668-783 bp	1.0	79.1
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	655-770 bp	1.0	78.6
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TTCAGTCCTCTGCTCTACCA	50.0	50.0	57.5	57.4	656-771 bp	1.0	78.6