Hotspot

trnS-UCC-rps4

LSC · 44265-44571 · 307 bp

Back to Rhammatophyllum

Pi 0.0315
MarkerSeek score 74.10
Primer available yes
Label trnS-UCC-rps4

Feature Metadata

label_name
trnS-UCC-rps4
feature_type
igs
region
LSC
length_bp
307
variable_sites
14
pi
0.0315
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UCC-rps4_p1 AGGCTTTTGTTTACCGAGGG AGGAAATGCGTCGGTTTGAA 50.0 45.0 58.1 58.4 413 bp 1.0 81.1
trnS-UCC-rps4_p2 AGGCTTTTGTTTACCGAGGG GGAAATGCGTCGGTTTGAAA 50.0 45.0 58.1 57.6 412 bp 1.0 81.0
trnS-UCC-rps4_p3 AGGCTTTTGTTTACCGAGGG TAGGAAATGCGTCGGTTTGA 50.0 45.0 58.1 57.5 414 bp 1.0 80.6
trnS-UCC-rps4_p4 GAGTGGTTGAAGGCGTAACA AGGAAATGCGTCGGTTTGAA 50.0 45.0 58.1 58.4 448 bp 1.0 80.4
trnS-UCC-rps4_p5 GAGTGGTTGAAGGCGTAACA GGAAATGCGTCGGTTTGAAA 50.0 45.0 58.1 57.6 447 bp 1.0 80.4