Hotspot

trnK-AAA-rps16

LSC · 4345-5035 · 691 bp

Back to Rhammatophyllum

Pi 0.0421
MarkerSeek score 78.60
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
691
variable_sites
37
pi
0.0421
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 AAGCCGAGTACTCTACCGTT AGGCAGGGATTTTTACAGAA 50.0 40.0 58.2 54.5 737-803 bp 1.0 58.6
trnK-AAA-rps16_p2 AAAGCCGAGTACTCTACCGT AAGGCAGGGATTTTTACAGA 50.0 40.0 58.2 54.5 739-805 bp 1.0 58.5
trnK-AAA-rps16_p3 AAAGCCGAGTACTCTACCGT AGGCAGGGATTTTTACAGAA 50.0 40.0 58.2 54.5 738-804 bp 1.0 58.5
trnK-AAA-rps16_p4 AAGCCGAGTACTCTACCGTT AAGGCAGGGATTTTTACAGA 50.0 40.0 58.2 54.5 738-804 bp 1.0 58.5
trnK-AAA-rps16_p5 AAGCCGAGTACTCTACCGTT AAGGCAGGGATTTTTACAGAA 50.0 38.1 58.2 55.4 738-804 bp 1.0 57.4