Hotspot

trnH-CAC-psbA

LSC · 77-388 · 312 bp

Back to Rhammatophyllum

Pi 0.0742

MarkerSeek score 79.00

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 33
pi: 0.0742
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.9	57.4	365-392 bp	0.666667	60.0
trnH-CAC-psbA_p2	ACTGCCTTAATCCACTTGGC	TGAGGCTCCATCTACAAATGG	50.0	47.6	58.2	57.4	366-393 bp	0.666667	59.8
trnH-CAC-psbA_p3	AATCCACTTGGCTACATCCG	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.7	57.4	358-385 bp	0.666667	58.6
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAGGCTCCATCTACAAATGG	50.0	45.5	57.9	58.1	366-393 bp	0.666667	55.7
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	TTGAGGCTCCATCTACAAATGG	50.0	45.5	58.2	58.1	367-394 bp	0.666667	55.4