Hotspot

rpl36-infA

LSC · 104517-104619 · 103 bp

Back to Reynoutria

Pi 0.0194

MarkerSeek score 63.70

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 103
variable_sites: 2
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ACTATAATTCGTCCCCGCCT	TGCCGGGGGATAGAGTTAAA	50.0	50.0	58.3	58.1	272 bp	1.0	77.4
rpl36-infA_p2	CAAATTTTACGCACGGAGGC	TGCCGGGGGATAGAGTTAAA	50.0	50.0	58.4	58.1	230 bp	1.0	76.7
rpl36-infA_p3	AAATTTTACGCACGGAGGCT	TGCCGGGGGATAGAGTTAAA	45.0	50.0	58.5	58.1	229 bp	1.0	76.0
rpl36-infA_p4	CGCACGGAGGCTCTTATTTT	TGCCGGGGGATAGAGTTAAA	50.0	50.0	58.6	58.1	221 bp	1.0	74.7
rpl36-infA_p5	ACTATAATTCGTCCCCGCCT	TTTATACGAATCCTGCCGGG	50.0	50.0	58.3	57.5	285 bp	1.0	74.0