Hotspot

psaA-ycf3

LSC · 65391-66118 · 728 bp

Back to Reynoutria

Pi 0.00695

MarkerSeek score 63.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 5
pi: 0.00695
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGGTTTTTACGGGGTCCCTA	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.0	57.8	855-864 bp	1.0	79.6
psaA-ycf3_p2	AGGTTTTTACGGGGTCCCTA	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.0	58.2	859-868 bp	1.0	79.4
psaA-ycf3_p3	AGGTTTTTACGGGGTCCCTA	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.0	58.3	854-863 bp	1.0	78.6
psaA-ycf3_p4	CGGGGTCCCTATCTACCAAA	TTGAAGATCACGAGGCGTTT	55.0	45.0	58.2	57.8	846-855 bp	1.0	78.4
psaA-ycf3_p5	AGGTTTTTACGGGGTCCCTA	TCACGAGGCGTTTCGAATAA	50.0	45.0	58.0	57.6	848-857 bp	1.0	78.2