Hotspot

psbM-trnD-GUC

LSC · 52171-53220 · 1050 bp

Back to Reynoutria

Pi 0.0267

MarkerSeek score 74.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 28
pi: 0.0267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGTTTTGGCTGACCGTTTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	57.0	58.5	1214 bp	1.0	69.7
psbM-trnD-GUC_p2	TTGTTTTGGCTGACCGTTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	57.0	58.5	1215 bp	1.0	69.7
psbM-trnD-GUC_p3	AATTGTTTTGGCTGACCGTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.7	58.5	1217 bp	1.0	67.9
psbM-trnD-GUC_p4	ATTGTTTTGGCTGACCGTTT	TTCAATTGGTCAGAGCACCG	40.0	50.0	56.7	58.5	1216 bp	1.0	67.9
psbM-trnD-GUC_p5	TTGTTTTGGCTGACCGTTTTT	TTCAATTGGTCAGAGCACCG	38.1	50.0	57.7	58.5	1215 bp	1.0	67.5