Hotspot

rpoB-trnC-GCA

LSC · 49179-50500 · 1322 bp

Back to Reynoutria

Pi 0.00681
MarkerSeek score 62.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1322
variable_sites
9
pi
0.00681
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GCCCAGGTATTGTCGACATT CCAGATTTGAACCGGGGAAA 50.0 50.0 58.0 58.1 1428-1441 bp 1.0 79.6
rpoB-trnC-GCA_p2 GCCCAGGTATTGTCGACATT AAAGGATTTGCAGTCCTCCG 50.0 50.0 58.0 58.2 1408-1421 bp 1.0 78.9
rpoB-trnC-GCA_p3 GTTCTTCTGTCAAGCCCTGA CCAGATTTGAACCGGGGAAA 50.0 50.0 57.4 58.1 1488-1501 bp 1.0 75.5
rpoB-trnC-GCA_p4 AGTTCTTCTGTCAAGCCCTG CCAGATTTGAACCGGGGAAA 50.0 50.0 57.4 58.1 1489-1502 bp 1.0 75.4
rpoB-trnC-GCA_p5 GTTCTTCTGTCAAGCCCTGA AAAGGATTTGCAGTCCTCCG 50.0 50.0 57.4 58.2 1468-1481 bp 1.0 74.7