Hotspot

atpF-atpH

LSC · 35594-36044 · 451 bp

Back to Reynoutria

Pi 0.00935

MarkerSeek score 65.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 4
pi: 0.00935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAATCCGAAACTCCCGGC	GCAGAGGGGAAAATACGAGG	50.0	55.0	58.3	58.0	614-633 bp	1.0	81.0
atpF-atpH_p2	CCCGGGGAAACGAAAGAATC	GCAGAGGGGAAAATACGAGG	55.0	55.0	58.9	58.0	581-600 bp	1.0	76.0
atpF-atpH_p3	TTAAATCCGAAACTCCCGGC	CCGAAGCAGAGGGGAAAATA	50.0	50.0	58.3	57.3	619-638 bp	1.0	75.7
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	AGCAGAGGGGAAAATACGAG	50.0	50.0	58.3	57.0	615-634 bp	1.0	73.5
atpF-atpH_p5	CCCGGGGAAACGAAAGAAT	GCAGAGGGGAAAATACGAGG	52.6	55.0	57.7	58.0	581-600 bp	1.0	73.2