Hotspot

trnK-UUU-rps16

LSC · 25441-26419 · 979 bp

Back to Reynoutria

Pi 0.00415
MarkerSeek score 62.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
979
variable_sites
4
pi
0.00415
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA CGCTCAACCTACTGGAACTG 50.0 55.0 58.0 58.3 1140-1149 bp 1.0 78.6
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC CGCTCAACCTACTGGAACTG 50.0 55.0 58.0 58.3 1141-1150 bp 1.0 78.6
trnK-UUU-rps16_p3 GGACGAATCCCTTGCTTCAT CGCTCAACCTACTGGAACTG 50.0 55.0 58.0 58.3 1143-1152 bp 1.0 78.6
trnK-UUU-rps16_p4 AACTCTACCGATGGTGTGGA CGCTCAACCTACTGGAACTG 50.0 55.0 58.1 58.3 1160-1169 bp 1.0 78.4
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA ATTTTCTTGAAAGGGGCGCT 50.0 45.0 58.0 58.4 1156-1165 bp 1.0 78.0