Hotspot

trnT-GGU-psbD

LSC · 31347-32703 · 1357 bp

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Pi 0.0422
MarkerSeek score 73.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1357
variable_sites
57
pi
0.0422
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1437-1475 bp 1.0 43.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1440-1478 bp 1.0 43.5
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1414-1452 bp 1.0 43.5
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1436-1474 bp 1.0 43.5
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1439-1477 bp 1.0 43.5