Hotspot

trnY-GUA-trnE-UUC

LSC · 30769-30816 · 48 bp

Back to Reseda

Pi 0.0833
MarkerSeek score 76.90
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
48
variable_sites
4
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CCAGCGTAGACATATTGCCA AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 192 bp 1.0 79.0
trnY-GUA-trnE-UUC_p2 ACCAGCGTAGACATATTGCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 193 bp 1.0 79.0
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC AAGTATGCCCCCATCGTCTA 55.0 50.0 58.4 57.9 171 bp 1.0 78.3
trnY-GUA-trnE-UUC_p4 ACGAATTTACAGTCCGTCCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.6 57.9 172 bp 1.0 78.1
trnY-GUA-trnE-UUC_p5 CCAGCGTAGACATATTGCCA CCATCGTCTAGTGGTCAGGA 50.0 55.0 57.8 58.2 182 bp 1.0 78.0