Hotspot

trnD-GUC-trnY-GUA

LSC · 30232-30682 · 451 bp

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Pi 0.0622
MarkerSeek score 81.30
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
451
variable_sites
27
pi
0.0622
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TATTAGGTCGGTCGGGACTG AAGCTACTTCCTGGGTCGAT 55.0 50.0 58.3 58.1 628-634 bp 1.0 82.0
trnD-GUC-trnY-GUA_p2 TATTAGGTCGGTCGGGACTG TGGCAATATGTCTACGCTGG 55.0 50.0 58.3 57.8 572-578 bp 1.0 81.7
trnD-GUC-trnY-GUA_p3 TATTAGGTCGGTCGGGACTG GGCAATATGTCTACGCTGGT 55.0 50.0 58.3 57.8 571-577 bp 1.0 81.7
trnD-GUC-trnY-GUA_p4 TTATTAGGTCGGTCGGGACT AAGCTACTTCCTGGGTCGAT 50.0 50.0 57.6 58.1 629-635 bp 1.0 81.0
trnD-GUC-trnY-GUA_p5 TATTAGGTCGGTCGGGACTG GGGGACGGACTGTAAATTCG 55.0 55.0 58.3 58.4 593-599 bp 1.0 80.6