Hotspot

psbM-trnD-GUC

LSC · 29193-30157 · 965 bp

Back to Reseda

Pi 0.0754
MarkerSeek score 84.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
965
variable_sites
67
pi
0.0754
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACAAGAATGAAGAGTGCA AGTTCAATTGGTCAGAGCGC 40.9 50.0 57.9 58.8 1029-1085 bp 1.0 65.6
psbM-trnD-GUC_p2 AGGCAGTAGGAACAAGAATGA AGTTCAATTGGTCAGAGCGC 42.9 50.0 56.6 58.8 1036-1092 bp 1.0 63.4
psbM-trnD-GUC_p3 GGAACAAGAATGAAGAGTGCAG AGTTCAATTGGTCAGAGCGC 45.5 50.0 57.6 58.8 1028-1084 bp 1.0 63.0
psbM-trnD-GUC_p4 TTGACTGACCGTTTTTACGT AGTTCAATTGGTCAGAGCGC 40.0 50.0 55.6 58.8 1071-1127 bp 1.0 62.7
psbM-trnD-GUC_p5 CTTTGACTGACCGTTTTTACGT AGTTCAATTGGTCAGAGCGC 40.9 50.0 57.5 58.8 1073-1129 bp 1.0 61.9