Hotspot

petN-psbM

LSC · 28291-29087 · 797 bp

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Pi 0.0442
MarkerSeek score 86.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
797
variable_sites
34
pi
0.0442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG TGCTACTGCACTCTTCATTCT 50.0 42.9 58.0 57.0 958-1029 bp 1.0 69.0
petN-psbM_p2 AGTAAATCTCGCTTGGGCTG TGCACTCTTCATTCTTGTTCCT 50.0 40.9 58.0 57.9 952-1023 bp 1.0 68.1
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTCTTCATTCT 50.0 42.9 57.6 57.0 898-969 bp 1.0 66.8
petN-psbM_p4 AGTAAATCTCGCTTGGGCTG TGCTACTGCACTCTTCATTCTT 50.0 40.9 58.0 57.7 958-1029 bp 1.0 66.5
petN-psbM_p5 AGTAAATCTCGCTTGGGCTG TCATTCTTGTTCCTACTGCCT 50.0 42.9 58.0 56.6 944-1015 bp 1.0 66.0