Hotspot

trnR-UCU-atpA

LSC · 9660-9810 · 151 bp

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Pi 0.0993
MarkerSeek score 77.00
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
151
variable_sites
15
pi
0.0993
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TCAGGAGCAAATGGAACGTT 47.6 45.0 57.3 57.7 216-228 bp 1.0 69.7
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TTCAGGAGCAAATGGAACGT 47.6 45.0 57.3 57.7 217-229 bp 1.0 69.7
trnR-UCU-atpA_p3 TGGATAGGACAGAGGTCTTCT TCAGGAGCAAATGGAACGTT 47.6 45.0 56.9 57.7 251-263 bp 1.0 66.1
trnR-UCU-atpA_p4 TGGATAGGACAGAGGTCTTCT TTCAGGAGCAAATGGAACGT 47.6 45.0 56.9 57.7 252-264 bp 1.0 66.1
trnR-UCU-atpA_p5 ACTAGAAAAGCGTCCATTGTCT TCAGGAGCAAATGGAACGTT 40.9 45.0 57.7 57.7 275-287 bp 1.0 63.9