Hotspot

trnN-GUU-rpl32

SSC · 109190-110005 · 816 bp

Back to Renanthera

Pi 0.0709
MarkerSeek score 83.30
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
816
variable_sites
87
pi
0.0709
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG TTTGACATAGAAGTACGTTTTT 50.0 27.3 58.1 52.0 699-1033 bp 1.0 52.0
trnN-GUU-rpl32_p2 AGTCAGTTAACAGCCAACCG TTTTGACATAGAAGTACGTTTT 50.0 27.3 58.1 52.0 700-1034 bp 1.0 52.0
trnN-GUU-rpl32_p3 AGTCAGTTAACAGCCAACCG TTTTTGACATAGAAGTACGTTT 50.0 27.3 58.1 52.0 701-1035 bp 1.0 52.0
trnN-GUU-rpl32_p4 ACGACCAGTCAGTTAACAGC TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.8 52.0 705-1039 bp 1.0 52.0
trnN-GUU-rpl32_p5 ACGACCAGTCAGTTAACAGC TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.8 52.0 706-1040 bp 1.0 52.0