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psbT-psbN

LSC · 74820-74881 · 62 bp

Back to Renanthera

Pi 0.0484

MarkerSeek score 75.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 6
pi: 0.0484
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	CCTTCGAAGAACATGGGGAC	47.6	55.0	57.6	58.3	124 bp	1.0	70.6
psbT-psbN_p2	TCGAGAACCACCTAAAGTTCC	CCTTCGAAGAACATGGGGAC	47.6	55.0	57.3	58.3	125 bp	1.0	68.4
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	ATCCCTTCGAAGAACATGGG	47.6	50.0	57.6	57.3	127 bp	1.0	66.9
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	CTTCGAAGAACATGGGGACT	47.6	50.0	57.6	57.2	123 bp	1.0	66.6
psbT-psbN_p5	TTCGAGAACCACCTAAAGTTCC	CCTTCGAAGAACATGGGGAC	45.5	55.0	58.0	58.3	126 bp	1.0	65.7