Hotspot

psbB-psbT

LSC · 74321-74711 · 391 bp

Back to Renanthera

Pi 0.0348

MarkerSeek score 72.70

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 23
pi: 0.0348
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	537-553 bp	1.0	71.1
psbB-psbT_p2	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	536-552 bp	1.0	68.8
psbB-psbT_p3	TCCAACTACAAGGAGACAGGT	TGGAACTTTAGGTGGTTCTCG	47.6	47.6	58.0	57.6	499-515 bp	1.0	68.2
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	GGAACTTTAGGTGGTTCTCGAA	50.0	45.5	57.4	58.0	536-552 bp	1.0	66.2
psbB-psbT_p5	TCCAACTACAAGGAGACAGGT	GGAACTTTAGGTGGTTCTCGA	47.6	47.6	58.0	57.3	498-514 bp	1.0	66.0