Hotspot

psbE-petL

LSC · 64564-65701 · 1138 bp

Back to Renanthera

Pi 0.0259

MarkerSeek score 73.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 51
pi: 0.0259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1130-1308 bp	1.0	78.8
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1126-1304 bp	1.0	78.8
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1128-1306 bp	1.0	78.8
psbE-petL_p4	ACTGACGAATAACCAACCCG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1139-1317 bp	1.0	78.5
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1141-1319 bp	1.0	78.5